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rabbit anti smmhc  (Proteintech)


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    Structured Review

    Proteintech rabbit anti smmhc
    Rabbit Anti Smmhc, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+smmhc/SMMHC+Antibody/pm41443304-142-63-65
    Average 94 stars, based on 108 article reviews
    rabbit anti smmhc - by Bioz Stars, 2026-09
    94/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Neonatal Streptococcus Pneumoniae pneumonia induces airway SMMHC expression through HMGB1/TLR4/ERK.
    Article Snippet: Background: Our previous study showed that neonatal S. pneumoniae pneumonia promoted airway smooth muscle myosin heavy chain (SMMHC) expression and AHR development.. Researches demonstrated HMGB1, TLR4 and ERK are involved in smooth muscle contractile protein expression, so we hypothesis that HMGB1/ TLR4/ERK pathway participated in airway SMMHC overexpression in neonatal S. pneumoniae pneumonia

    Article Title: Ephrin-A1/EphA2 modulates vascular smooth muscle cell proliferation in vitro, while VSMC-specific ephrin-A1 deletion is not sufficient to alter arteriosclerosis in vivo.
    Article Snippet: Immunohistochemical staining were done using the Dako EnVision® + System–HRP (AEC) (Dako, Germany). .. Target retrieval was done by boiling with appropriate buffer and subsequent slow cooling for 30 min. Endogenous peroxidase activity was quenched with peroxidase block (Dako, Germany) for 10 min. Incubation with protein blocking solution (Dako, Germany) was performed for 20 min, and sections were subsequently incubated with primary antibodies diluted in antibody diluent solution (Dako, Germany) for 1 h. Following antibodies were used: rabbit anti-SMMHC (Proteintech, #21404-1-AP, 1:100), rabbit anti-GAL3 (Sigma-Aldrich, #SAB4501746, 1:100), rabbit anti-PCNA (Sigma-Aldrich, #HPA030522, 1:50), rabbit anti-EphA2 (Cell Signaling Technologies, #6997S, 1:50), and rabbit anti-CD45 (Cell Signaling Technologies, #70257, 1:30). .. Thereafter an anti-rabbit antibody conjugated to HRP-labelled polymer was applicated for 45 min. Color development was done with AEC+ substrate (Dako, Germany).

    Activity Assay:

    Article Title: Ephrin-A1/EphA2 modulates vascular smooth muscle cell proliferation in vitro, while VSMC-specific ephrin-A1 deletion is not sufficient to alter arteriosclerosis in vivo.
    Article Snippet: Immunohistochemical staining were done using the Dako EnVision® + System–HRP (AEC) (Dako, Germany). .. Target retrieval was done by boiling with appropriate buffer and subsequent slow cooling for 30 min. Endogenous peroxidase activity was quenched with peroxidase block (Dako, Germany) for 10 min. Incubation with protein blocking solution (Dako, Germany) was performed for 20 min, and sections were subsequently incubated with primary antibodies diluted in antibody diluent solution (Dako, Germany) for 1 h. Following antibodies were used: rabbit anti-SMMHC (Proteintech, #21404-1-AP, 1:100), rabbit anti-GAL3 (Sigma-Aldrich, #SAB4501746, 1:100), rabbit anti-PCNA (Sigma-Aldrich, #HPA030522, 1:50), rabbit anti-EphA2 (Cell Signaling Technologies, #6997S, 1:50), and rabbit anti-CD45 (Cell Signaling Technologies, #70257, 1:30). .. Thereafter an anti-rabbit antibody conjugated to HRP-labelled polymer was applicated for 45 min. Color development was done with AEC+ substrate (Dako, Germany).

    Blocking Assay:

    Article Title: Ephrin-A1/EphA2 modulates vascular smooth muscle cell proliferation in vitro, while VSMC-specific ephrin-A1 deletion is not sufficient to alter arteriosclerosis in vivo.
    Article Snippet: Immunohistochemical staining were done using the Dako EnVision® + System–HRP (AEC) (Dako, Germany). .. Target retrieval was done by boiling with appropriate buffer and subsequent slow cooling for 30 min. Endogenous peroxidase activity was quenched with peroxidase block (Dako, Germany) for 10 min. Incubation with protein blocking solution (Dako, Germany) was performed for 20 min, and sections were subsequently incubated with primary antibodies diluted in antibody diluent solution (Dako, Germany) for 1 h. Following antibodies were used: rabbit anti-SMMHC (Proteintech, #21404-1-AP, 1:100), rabbit anti-GAL3 (Sigma-Aldrich, #SAB4501746, 1:100), rabbit anti-PCNA (Sigma-Aldrich, #HPA030522, 1:50), rabbit anti-EphA2 (Cell Signaling Technologies, #6997S, 1:50), and rabbit anti-CD45 (Cell Signaling Technologies, #70257, 1:30). .. Thereafter an anti-rabbit antibody conjugated to HRP-labelled polymer was applicated for 45 min. Color development was done with AEC+ substrate (Dako, Germany).



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    Immunohistochemistry and immunoblotting of vascular smooth muscle phenotypic markers in the aortic media. Hematoxylin-and-eosin–stained sections of the aortic media ( A ). Immunohistochemistry revealed a significantly decreased positive area of smooth muscle myosin heavy chain <t>(SM-MHC)</t> ( B ) and smoothelin ( C ) immunostaining, and an increased positive area for S100A4 immunostaining ( D ) in the dissected media compared with control aortic media. Immunoblotting revealed a tendency similar to that observed by immunohistochemistry ( E-G ). See for the original Western blotting images. Scale bars = 200 μm.
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    Immunohistochemistry and immunoblotting of vascular smooth muscle phenotypic markers in the aortic media. Hematoxylin-and-eosin–stained sections of the aortic media ( A ). Immunohistochemistry revealed a significantly decreased positive area of smooth muscle myosin heavy chain <t>(SM-MHC)</t> ( B ) and smoothelin ( C ) immunostaining, and an increased positive area for S100A4 immunostaining ( D ) in the dissected media compared with control aortic media. Immunoblotting revealed a tendency similar to that observed by immunohistochemistry ( E-G ). See for the original Western blotting images. Scale bars = 200 μm.
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    Immunohistochemistry and immunoblotting of vascular smooth muscle phenotypic markers in the aortic media. Hematoxylin-and-eosin–stained sections of the aortic media ( A ). Immunohistochemistry revealed a significantly decreased positive area of smooth muscle myosin heavy chain <t>(SM-MHC)</t> ( B ) and smoothelin ( C ) immunostaining, and an increased positive area for S100A4 immunostaining ( D ) in the dissected media compared with control aortic media. Immunoblotting revealed a tendency similar to that observed by immunohistochemistry ( E-G ). See for the original Western blotting images. Scale bars = 200 μm.
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    Neonatal S. pneumoniae pneumonia alters airway smooth muscle productions in mice model. IHC staining of lung tissues from both mock-infected (control) and neonatal S. pneumoniae pneumonia mice (S.pp) showing the in situ expression <t>of</t> <t>α</t> -smooth muscle actin ( α -SMA) (a), smooth muscle myosin heavy chain <t>(SMMHC)</t> (f), and smooth muscle 22 alpha (SM22 α ) (k) (400x magnification). The relative α -SMA-positive ( α -SMA + area/Pbm 2 ) (b), SMMHC-positive (SMMHC + area/Pbm 2 ) (g), and SM22 α -positive (SM22 α + area/Pbm 2 ) (l) areas are also shown. RT-qPCR was used to analyze Acta2-mRNA (c), Myh11-mRNA (h), and Tagln-mRNA (m) levels in the lung tissues. The α -SMA (d, e), SMMHC (i, j), and SM22 α (n, o) protein levels in the lung tissues were analyzed by Western blotting. All data are presented as means ± SD. (n =5/group). ∗∗ P<0.01, compared to the control group.
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    Image Search Results


    Immunohistochemistry and immunoblotting of vascular smooth muscle phenotypic markers in the aortic media. Hematoxylin-and-eosin–stained sections of the aortic media ( A ). Immunohistochemistry revealed a significantly decreased positive area of smooth muscle myosin heavy chain (SM-MHC) ( B ) and smoothelin ( C ) immunostaining, and an increased positive area for S100A4 immunostaining ( D ) in the dissected media compared with control aortic media. Immunoblotting revealed a tendency similar to that observed by immunohistochemistry ( E-G ). See for the original Western blotting images. Scale bars = 200 μm.

    Journal: CJC Open

    Article Title: Relationship Between Vascular Smooth Muscle Cell Phenotype and Degeneration of Elastin in the Aortic Media in Patients With Acute Aortic Dissection

    doi: 10.1016/j.cjco.2025.03.020

    Figure Lengend Snippet: Immunohistochemistry and immunoblotting of vascular smooth muscle phenotypic markers in the aortic media. Hematoxylin-and-eosin–stained sections of the aortic media ( A ). Immunohistochemistry revealed a significantly decreased positive area of smooth muscle myosin heavy chain (SM-MHC) ( B ) and smoothelin ( C ) immunostaining, and an increased positive area for S100A4 immunostaining ( D ) in the dissected media compared with control aortic media. Immunoblotting revealed a tendency similar to that observed by immunohistochemistry ( E-G ). See for the original Western blotting images. Scale bars = 200 μm.

    Article Snippet: Rabbit polyclonal antibodies against SM-MHC (#21404-1-AP; Proteintech, Rosemont, IL), S100A4 (#A5114; Agilent Technologies, Santa Clara, CA), and elastin (#15257-1-AP; Proteintech) were used.

    Techniques: Immunohistochemistry, Western Blot, Staining, Immunostaining, Control

    The relationship between each protein expression level. The levels of 2 well-differentiated vascular smooth muscle cell (VSMC) markers, SM-MHC, and smoothelin, correlated well ( A ). S100A4 levels were negatively correlated with smoothelin ( B ) and SM-MHC ( C ) levels. A significant positive correlation was observed between the distribution of elastin and smoothelin ( D ), and SM-MHC ( E ) levels. A negative correlation was observed between elastin and S100A4 levels, but it was not statistically significant ( F ). ρ, correlation coefficient.

    Journal: CJC Open

    Article Title: Relationship Between Vascular Smooth Muscle Cell Phenotype and Degeneration of Elastin in the Aortic Media in Patients With Acute Aortic Dissection

    doi: 10.1016/j.cjco.2025.03.020

    Figure Lengend Snippet: The relationship between each protein expression level. The levels of 2 well-differentiated vascular smooth muscle cell (VSMC) markers, SM-MHC, and smoothelin, correlated well ( A ). S100A4 levels were negatively correlated with smoothelin ( B ) and SM-MHC ( C ) levels. A significant positive correlation was observed between the distribution of elastin and smoothelin ( D ), and SM-MHC ( E ) levels. A negative correlation was observed between elastin and S100A4 levels, but it was not statistically significant ( F ). ρ, correlation coefficient.

    Article Snippet: Rabbit polyclonal antibodies against SM-MHC (#21404-1-AP; Proteintech, Rosemont, IL), S100A4 (#A5114; Agilent Technologies, Santa Clara, CA), and elastin (#15257-1-AP; Proteintech) were used.

    Techniques: Expressing

    Neonatal S. pneumoniae pneumonia alters airway smooth muscle productions in mice model. IHC staining of lung tissues from both mock-infected (control) and neonatal S. pneumoniae pneumonia mice (S.pp) showing the in situ expression of α -smooth muscle actin ( α -SMA) (a), smooth muscle myosin heavy chain (SMMHC) (f), and smooth muscle 22 alpha (SM22 α ) (k) (400x magnification). The relative α -SMA-positive ( α -SMA + area/Pbm 2 ) (b), SMMHC-positive (SMMHC + area/Pbm 2 ) (g), and SM22 α -positive (SM22 α + area/Pbm 2 ) (l) areas are also shown. RT-qPCR was used to analyze Acta2-mRNA (c), Myh11-mRNA (h), and Tagln-mRNA (m) levels in the lung tissues. The α -SMA (d, e), SMMHC (i, j), and SM22 α (n, o) protein levels in the lung tissues were analyzed by Western blotting. All data are presented as means ± SD. (n =5/group). ∗∗ P<0.01, compared to the control group.

    Journal: BioMed Research International

    Article Title: Neonatal Streptococcus pneumoniae Pneumonia Induces an Aberrant Airway Smooth Muscle Phenotype and AHR in Mice Model

    doi: 10.1155/2019/1948519

    Figure Lengend Snippet: Neonatal S. pneumoniae pneumonia alters airway smooth muscle productions in mice model. IHC staining of lung tissues from both mock-infected (control) and neonatal S. pneumoniae pneumonia mice (S.pp) showing the in situ expression of α -smooth muscle actin ( α -SMA) (a), smooth muscle myosin heavy chain (SMMHC) (f), and smooth muscle 22 alpha (SM22 α ) (k) (400x magnification). The relative α -SMA-positive ( α -SMA + area/Pbm 2 ) (b), SMMHC-positive (SMMHC + area/Pbm 2 ) (g), and SM22 α -positive (SM22 α + area/Pbm 2 ) (l) areas are also shown. RT-qPCR was used to analyze Acta2-mRNA (c), Myh11-mRNA (h), and Tagln-mRNA (m) levels in the lung tissues. The α -SMA (d, e), SMMHC (i, j), and SM22 α (n, o) protein levels in the lung tissues were analyzed by Western blotting. All data are presented as means ± SD. (n =5/group). ∗∗ P<0.01, compared to the control group.

    Article Snippet: The membranes were incubated with mouse anti- α -SMA (1:500; Sigma), rabbit anti-SMMHC (1:1000; Sigma), rabbit anti-SM22 α (1:1000; Sigma), or rabbit anti-GAPDH (1:1000; Proteintech) antibody for 12 h at 4°C.

    Techniques: Immunohistochemistry, Infection, In Situ, Expressing, Quantitative RT-PCR, Western Blot